HETEROGENEITY

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The majority of clinically important antibiotics that are used to combat bacterial infections are derived from actinomycetes. In addition to antimicrobials, these organisms produce numerous enzymes for medical and technical applications. My team investigates how we can improve growth of these prolific bacteria for industrial applications. We therefore focus on cellular and physiological heterogeneity . Heterogeneity is observed between clonal mycelial particles, between different zones of colonies, between adjacent hyphae and even between adjacent compartments of individual hyphae. By dissecting the mechanisms underlying heterogeneity we aim to identify new targets to improve the exploitability of these bacteria as cell factories in the biotech sector.

SELECTED PUBLICATIONS

  • Z. Zhang, F. de Barsy, M. Liem, A. Liakopoulos, Y.H. Choi, D. Claessen & D.E. Rozen (2019). Antibiotic production is organised by a division of labour in Streptomyces. bioRxiv: 560136; doi: https://doi.org/10.1101/560136.
  • B. Zacchetti, H.A.B. Wösten & D. Claessen (2018). Multiscale heterogeneity in filamentous microbes. Biotech Adv 36: 2138-2149.
  • K. Ramijan, E. Ultee, J. Willemse, Z. Zhang, J.A.J. Wondergem, A. van der Meij, D. Heinrich, A. Briegel, G.P. van Wezel & D. Claessen (2018). Stress-induced formation of cell wall-deficient cells in filamentous actinomycetes. Nat Commun 9: 5164. 
  • B. Zacchetti, P. Smits & D. Claessen (2018). Dynamics of pellet fragmentation and aggregation in liquid-grown cultures of Streptomyces lividans. Front Microbiol 9: 943.
  • B. Zacchetti, J. Willemse, B. Recter, D. van Dissel, G.P. van Wezel, H.A.B. Wösten & D. Claessen (2016). Aggregation of germlings is a major contributing factor towards mycelial heterogeneity of Streptomyces. Sci Rep 6: 27045.
  • G.J. van Veluw, M.L.C. Petrus, J. Gubbens, R. de Graaf, I.P. de Jong, G.P. van Wezel, H.A.B. Wösten & D. Claessen (2012). Analysis of two distinct mycelial populations in liquid-grown Streptomyces cultures using a flow cytometry-based proteomics approach. Appl Microbiol Biotechnol 96: 1301-1312.

© DENNIS CLAESSEN, 2019